1.Fluorescence "turn-on" determination of H2O2 using multilayer porous SiO2/NGQDs and PdAu mimetics enzymatic/oxidative cleavage of single-stranded DNA.
Liang L1, Lan F1, Li L1, Su M1, Ge S2, Yu J1, Liu H1, Yan M1. Biosens Bioelectron. 2016 Apr 1;82:204-211. doi: 10.1016/j.bios.2016.03.076. [Epub ahead of print]
A 3D microfluidic paper-based fluorescence analytical device with hollow channels based on the turn-on switching of a resonance energy transfer triggered by the •OH induced cleavage of a DNA strand was successfully constructed. And this fluorescent nanoplatform was first designed to achieve in situ and real-time determination of H2O2 released from cancer cells to obtain an accurate determination. With optimal conditions, the proposed method displayed excellent analytical performance for the detection of H2O2 ranging from 0.3 to 1.0mM with a detection limit of 0.1nM. The favorable performances of this sensor were due to the peroxidase-like activity of nitrogen-doped graphene quantum dots (multilayer porous SiO2 act as stabilizer to load more nitrogen-doped graphene quantum dots for signal amplification) and folic acid-pPdAu/GO (which also could act as an efficient fluorescence quencher and a recognition element of cancer cells by folic acid).
2.Spiral crack patterns observed for melt-grown spherulites of poly(L-lactic acid) upon quenching.
Matsuda F1, Sobajima T1, Irie S2, Sasaki T3. Eur Phys J E Soft Matter. 2016 Apr;39(4):41. doi: 10.1140/epje/i2016-16041-6. Epub 2016 Apr 19.
In this paper, we demonstrate the characteristic spiral cracking that appears on the surface of melt-grown poly(L-lactic acid) (PLLA) spherulites with relatively large sizes (greater than 0.4mm in diameter). The crack occurs via thermal shrinkage upon quenching after crystallization. Although concentric cracks on polymer spherulites have been found to occur in quite a few studies, spiral crack patterns have never been reported so far. The present spiral crack was observed for thick spherulites (> 10 μm), whereas the concentric crack pattern was frequently observed for thin spherulites (typically 5μm). The present PLLA spherulites exhibited a non-banded structure with no apparent structural periodicity at least on the scale of the spiral pitch, and thus no direct correlation between the crack pattern and the spherulitic structure was suggested. The spiral was revealed to be largely Archimedean of which the spiral pitch increases with an increase in the thickness of the spherulite.
3.Parathyroid hormone levels and high-residual platelet reactivity in patients receiving dual antiplatelet therapy with acetylsalicylic acid and clopidogrel or ticagrelor.
Verdoia M1, Pergolini P2, Rolla R2, Nardin M1, Barbieri L1, Schaffer A1, Bellomo G2, Marino P1, Suryapranata H1, De Luca G1; Novara Atherosclerosis Study Group (NAS). Cardiovasc Ther. 2016 Apr 17. doi: 10.1111/1755-5922.12188. [Epub ahead of print]
BACKGROUND: High on treatment platelet reactivity still represents a challenging issue, potentially vanishing the benefits of dual antiplatelet treatment in patients with coronary artery disease. However, very few is known on the determinants of suboptimal response to antiplatelet agents. Recent interests have emerged on the potential protrombotic effect of parathyroid hormone (PTH). Therefore, the aim of the present study was to assess the impact of parathyroid hormone (PTH) on platelet reactivity in patients receiving DAPT after an acute coronary syndrome or PCI.
4.Mesenchymal stem cell proliferation and mineralization but not osteogenic differentiation are strongly affected by extracellular pH.
Fliefel R1, Popov C2, Tröltzsch M3, Kühnisch J4, Ehrenfeld M3, Otto S3. J Craniomaxillofac Surg. 2016 Mar 19. pii: S1010-5182(16)00081-0. doi: 10.1016/j.jcms.2016.03.003. [Epub ahead of print]
Osteomyelitis is a serious complication in oral and maxillofacial surgery affecting bone healing. Bone remodeling is not only controlled by cellular components but also by ionic and molecular composition of the extracellular fluids in which calcium phosphate salts are precipitated in a pH dependent manner.