1.Effect of dietary lignocellulose on ileal and total tract digestibility of fat and fatty acids in broiler chickens.
Bogusławska-Tryk M1, Piotrowska A1, Szymeczko R1, Burlikowska K1. J Anim Physiol Anim Nutr (Berl). 2016 Apr 14. doi: 10.1111/jpn.12476. [Epub ahead of print]
The study was conducted to determine the effect of a lignocellulose supplemented diet on apparent ileal and total tract digestibility of fat and fatty acids (FA) in broiler chickens. A total of 48 21-day-old male Ross 308 chickens were divided into four treatment groups (n = 12) with six replicates per treatment. From 21 to 42 days of age, the broilers were fed experimental diets varied in the amount of lignocellulose: 0%, 0.25%, 0.5% and 1%. Total excreta were gathered during the last 3 days of the feeding trial and digesta was collected from the ileum at 42 days of the bird age. Digestibility was determined by the indicator method. The ether extract content in diet/digesta/excreta was determined by the gravimetric method, and fatty acid methyl esters were analysed by gas chromatography-mass spectrometry. Fat digestibility measured to the end of the small intestine and in the whole gastrointestinal tract in birds was high and exceeded 90% and 87% respectively.
2.Vascular Actions of Angiotensin 1-7 in the Human Microcirculation: Novel Role for Telomerase.
Durand MJ1, Zinkevich NS1, Riedel M1, Gutterman DD1, Nasci VL1, Salato VK1, Hijjawi JB1, Reuben CF1, North PE1, Beyer AM2. Arterioscler Thromb Vasc Biol. 2016 Apr 14. pii: ATVBAHA.116.307518. [Epub ahead of print]
OBJECTIVE: This study examined vascular actions of angiotensin 1-7 (ANG 1-7) in human atrial and adipose arterioles.
3.Comparison and validation of 2 analytical methods for the determination of free fatty acids in dairy products by gas chromatography with flame ionization detection.
Mannion DT1, Furey A2, Kilcawley KN3. J Dairy Sci. 2016 Apr 13. pii: S0022-0302(16)30178-3. doi: 10.3168/jds.2015-10795. [Epub ahead of print]
Accurate quantification of free fatty acids (FFA) in dairy products is important for quality control, nutritional, antimicrobial, authenticity, legislative, and flavor purposes. In this study, the performance of 2 widely used gas chromatographic flame ionization detection methods for determination of FFA in dairy products differing in lipid content and degree of lipolysis were evaluated. We used a direct on-column approach where the isolated FFA extract was injected directly and a derivatization approach where the FFA were esterified in the injector to methyl esters using tetramethylammonium hydroxide as a catalyst. A comprehensive validation was undertaken to establish method linearity, limits of detection, limits of quantification, accuracy, and precision. Linear calibrations of 3 to 700 mg/L (R2 > 0.999) and 20 to 700 mg/L (R2 > 0.997), and limits of detection and limits of quantification of 0.7 and 3 mg/L and 5 and 20 mg/L were obtained for the direct injection on-column and the derivatization method, respectively.
4.Density functional study of the electronic structure of dye-functionalized fullerenes and their model donor-acceptor complexes containing P3HT.
Baruah T1, Garnica A1, Paggen M1, Basurto L1, Zope RR1. J Chem Phys. 2016 Apr 14;144(14):144304. doi: 10.1063/1.4944469.
We study the electronic structure of C60 fullerenes functionalized with a thiophene-diketo-pyrrolopyrrole-thiophene based chromophore using density functional theory combined with large polarized basis sets. As the attached chromophore has electron donor character, the functionalization of the fullerene leads to a donor-acceptor (DA) system. We examine in detail the effect of the linker and the addition site on the electronic structure of the functionalized fullerenes. We further study the electronic structure of these DA complexes with a focus on the charge transfer excitations. Finally, we examine the interface of the functionalized fullerenes with the widely used poly(3-hexylthiophene-2,5-diyl) (P3HT) donor. Our results show that all functionalized fullerenes with an exception of the C60-pyrrolidine [6,6], where the pyrrolidine is attached at a [6,6] site, have larger electron affinities relative to the pristine C60 fullerene. We also estimate the quasi-particle gap, lowest charge transfer excitation energy, and the exciton binding energies of the functionalized fullerene-P3MT model systems.