2,4-Dioxohexahydropyrimidine-5-carboxylic acid - CAS 92264-75-0
Catalog number: 92264-75-0
Category: Main Product
Molecular Formula:
C5H6N2O4
Molecular Weight:
158.11
COA:
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Purity:
95%
MSDS:
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Melting Point:
255-256ºC
Density:
1.523
1.Preparation and characterization of affinity sorbents based on isoalloxazine-like ligands for separation of flavoenzymes.
Xin Y1, Yang H, Xiao X, Zhang L, Zhang Y, Tong Y, Wang W. J Sep Sci. 2011 Nov;34(21):2940-9. doi: 10.1002/jssc.201100474. Epub 2011 Sep 21.
Affinity ligands for flavoenzymes were synthesized based on the natural structure of flavo-coenzymes. Two typical flavoenzymes, cholesterol oxidase from Brevibacterium sp. and xanthine oxidase from bovine milk, were employed as standard enzymes. Fluorescent probes were synthesized from eight isoalloxazine-like chemicals and 5-aminofluorescein. Probe-enzyme interactions were analyzed via fluorescence spectra. Chemicals with high binding abilities to flavoenzymes were coupled with Sepharose through spacers composed of epichlorohydrin, ethylenediamine, 1,3-diaminopropane, 2-hydroxy-1,3-diaminopropane, and 1,4-diaminobutane, and subjected to adsorption analysis with flavoenzymes. The results indicated that ligands synthesized from 2,4-dioxohexahydropyrimidine-5-carboxylic acid, cytosine, 7-chloroalloxazine, and 8-chloroalloxazine had high binding abilities to the flavoenzymes. The affinity sorbent based on these ligands revealed a high theoretical maximum adsorption (Q(max)).
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CAS 92264-75-0 2,4-Dioxohexahydropyrimidine-5-carboxylic acid

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