1.Bicarbonate Values for Healthy Residents Living in Cities Above 1500 Meters of Altitude: A Theoretical Model and Systematic Review.
Ramirez-Sandoval JC1, Castilla-Peón MF2, Gotés-Palazuelos J1, Vázquez-García JC3, Wagner MP4, Merelo-Arias CA2, Vega-Vega O1,5, Rincón-Pedrero R1, Correa-Rotter R1. High Alt Med Biol. 2016 Apr 27. [Epub ahead of print]
Ramirez-Sandoval, Juan C., Maria F. Castilla-Peón, José Gotés-Palazuelos, Juan C. Vázquez-García, Michael P. Wagner, Carlos A. Merelo-Arias, Olynka Vega-Vega, Rodolfo Rincón-Pedrero, and Ricardo Correa-Rotter. Bicarbonate values for healthy residents living in cities above 1500 m of altitude: a theoretical model and systematic review. High Alt Med Biol 00:000-000, 2016-Plasma bicarbonate (HCO3-) concentration is the main value used to assess the metabolic component of the acid-base status. There is limited information regarding plasma HCO3- values adjusted for altitude for people living in cities at high altitude defined as 1500 m (4921 ft) or more above sea level. Our aim was to estimate the plasma HCO3- concentration in residents of cities at these altitudes using a theoretical model and compare these values with HCO3- values found on a systematic review, and with those venous CO2 values obtained in a sample of 633 healthy individuals living at an altitude of 2240 m (7350 ft).
2.Identification of lipid-phosphatidylserine (PS) as the target of unbiasedly selected cancer specific peptide-peptoid hybrid PPS1.
Desai TJ1, Toombs JE2, Minna JD2,3,4,5, Brekken RA2,3,4,6, Udugamasooriya DG1,7. Oncotarget. 2016 Apr 22. doi: 10.18632/oncotarget.8929. [Epub ahead of print]
Phosphatidylserine (PS) is an anionic phospholipid maintained on the inner-leaflet of the cell membrane and is externalized in malignant cells. We previously launched a careful unbiased selection targeting biomolecules (e.g. protein, lipid or carbohydrate) distinct to cancer cells by exploiting HCC4017 lung cancer and HBEC30KT normal epithelial cells derived from the same patient, identifying HCC4017 specific peptide-peptoid hybrid PPS1. In this current study, we identified PS as the target of PPS1. We validated direct PPS1 binding to PS using ELISA-like assays, lipid dot blot and liposome based binding assays. In addition, PPS1 recognized other negatively charged and cancer specific lipids such as phosphatidic acid, phosphatidylinositol and phosphatidylglycerol. PPS1 did not bind to neutral lipids such as phosphatidylethanolamine found in cancer and phosphatidylcholine and sphingomyelin found in normal cells. Further we found that the dimeric version of PPS1 (PPS1D1) displayed strong cytotoxicity towards lung cancer cell lines that externalize PS, but not normal cells.
3.Comparison of multi-enzyme and thermophilic bacteria on the hydrolysis of mariculture organic waste (MOW).
Guo L1, Sun M2, Zong Y2, Zhao Y2, Gao M2, She Z2. Water Sci Technol. 2016 Apr;73(8):1978-1985.
Mariculture organic waste (MOW) is rich in organic matter, which is a potential energy resource for anaerobic digestion. In order to enhance the anaerobic fermentation, the MOW was hydrolyzed by multi-enzyme and thermophilic bacteria. It was advantageous for soluble chemical oxygen demand (SCOD) release at MOW concentrations of 6 and 10 g/L with multi-enzyme and thermophilic bacteria pretreatments. For multi-enzyme, the hydrolysis was not obvious at substrate concentrations of 1 and 3 g/L, and the protein and carbohydrate increased with hydrolysis time at substrate concentrations of 6 and 10 g/L. For thermophilic bacteria, the carbohydrate was first released at 2-4 h and then consumed, and the protein increased with hydrolysis time. The optimal enzyme hydrolysis for MOW was determined by measuring the changes of SCOD, protein, carbohydrate, ammonia and total phosphorus, and comparing with acid and alkaline pretreatments.
4.Continuous treatment of flotation collector wastewater using a membrane bioreactor.
Lin W1, Dai Y1, Wu C2, Xu P1, Ren J1, Sun S1, Li B1. Water Sci Technol. 2016 Apr;73(8):1901-1909.
Aniline aerofloat (DDA) is a widely used material in China and has become a main pollutant in floatation wastewater. In this study, a membrane reactor (MBR) was constructed to continuously treat simulated wastewater contaminated with DDA. The study investigated the hydraulic retention time (HRT) and the impact of influent DDA concentration on MBR performance, and analyzed intermediates from the DDA biodegradation pathway and activated sludge transfer pathway. The results showed that a 3 h HRT was an efficient and economical time period for MBR to remove 95 ± 5 mg/L DDA from the simulated wastewater; the chemical oxygen demand reduction rate was 89.9%. DDA concentration negatively impacted MBR performance. MBR performance fluctuated slightly when HRT was 3 h, dissolved oxygen ranged from 4.8 to 5.3 mg/L, pH was between 6.5 and 7.0, and DDA concentrations were at 95 ± 5 mg/L DDA. The transfer pathway in the activated sludge of DDA was through soluble microbial products, loosely bound extracellular polymeric substances, tightly bound extracellular polymeric substances, and finally cell biodegradation.