1.Amino acid composition analysis of human secondary transport proteins and implications for reliable membrane topology prediction.
Saidijam M1, Azizpour S1, Patching SG2. J Biomol Struct Dyn. 2016 May 9:1-63. [Epub ahead of print]
Secondary transporters in humans are a large group of proteins that transport a wide range of ions, metals, organic and inorganic solutes involved in energy transduction, control of membrane potential and osmotic balance, metabolic processes and in the absorption or efflux of drugs and xenobiotics. They are also emerging as important targets for development of new drugs and as target sites for drug delivery to specific organs or tissues. We have performed amino acid composition and phylogenetic analyses and membrane topology predictions for 336 human secondary transport proteins and used the results to confirm protein classification and to look for trends and correlations with structural domains and specific substrates and/or function. Some proteins showed statistically high contents of individual amino acids or of groups of amino acids with similar physicochemical properties. One recurring trend was a correlation between high contents of charged and/or polar residues with misleading results in predictions of membrane topology, which was especially prevalent in Mitochondrial Carrier family proteins.
2.Inspiration from the mirror: D-amino acid containing peptides in biomedical approaches.
Feng Z, Xu B. Biomol Concepts. 2016 May 9. pii: /j/bmc.ahead-of-print/bmc-2015-0035/bmc-2015-0035.xml. doi: 10.1515/bmc-2015-0035. [Epub ahead of print]
D-amino acids, the enantiomers of naturally abundant L-amino acids, bear unique stereochemistry properties that lead to the resistance towards most of the endogenous enzymes. Previous works have demonstrated applications of D-amino acids in therapeutic development with the aid of mirror-image phage display and retro-inverso peptide synthesis. In this review, we highlight the recent progress and challenges in the exploration of D-amino acids at the interface of chemistry and life science. First, we will introduce some progress made in traditional application of D-amino acids to enhance biostability of peptide therapeutics. Then, we discuss some works that explore the relatively underexplored interactions between the enzyme and D-amino acids and enzymatic reactions of D-amino acids. To highlight the enzymatic reactions of D-amino acids, we will describe several emerging works on the enzyme-instructed self-assembly (EISA) and their potential application in selective anti-inflammatory or anticancer therapies.
3.Six Month Clinical Evaluation of Interdental Papilla Reconstruction with Injectable Hyaluronic Acid Gel Using an Image Analysis System.
Lee WP1, Kim HJ2, Yu SJ3, Kim BO4. J Esthet Restor Dent. 2016 May 9. doi: 10.1111/jerd.12216. [Epub ahead of print]
PURPOSE: Obtaining predictable and aesthetically pleasing interdental papilla is challenging in dental reconstruction. Hyaluronic acid gel has been successfully used to reduce facial creases and similar abnormalities. The purpose of this study was to clinically assess the efficiency of interdental papilla reconstruction with injectable hyaluronic acid gel.
4.Eicosapentaenoic acid and arachidonic acid differentially regulate adipogenesis, acquisition of a brite phenotype and mitochondrial function in primary human adipocytes.
Fleckenstein-Elsen M1,2, Dinnies D1,2, Jelenik T2,3, Roden M2,3,4, Romacho T1,2, Eckel J1,2. Mol Nutr Food Res. 2016 May 9. doi: 10.1002/mnfr.201500892. [Epub ahead of print]
SCOPE: n-3 and n-6 PUFAs have several opposing biological effects and influence white adipose tissue (WAT) function. The recent discovery of thermogenic UCP1-expressing brite adipocytes within WAT raised the question whether n-3 and n-6 PUFAs exert differential effects on brite adipocyte formation and mitochondrial function.