1,3-a-1,6-a-D-Mannotriose - CAS 121123-33-9
Category:
Carbohydrates
Product Name:
1,3-a-1,6-a-D-Mannotriose
Synonyms:
Man-a-1-3-(Man-a-1-6)-Man; 3,6-Di-O-(a-D-mannopyranosyl)-D-mannopyranose
CAS Number:
121123-33-9
Molecular Weight:
504.44
Molecular Formula:
C18H32O16
COA:
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MSDS:
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Structure:
Oligosaccharides
Chemical Structure
CAS 121123-33-9 1,3-a-1,6-a-D-Mannotriose

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Reference Reading


1.Structural characterization of a mannose-binding protein-trimannoside complex using residual dipolar couplings.
Jain NU1, Noble S, Prestegard JH. J Mol Biol. 2003 Apr 25;328(2):451-62.
The ligand-binding properties of a 53 kDa homomultimeric trimer from mannose-binding protein (MBP) have been investigated using residual dipolar couplings (RDCs) that are easily measured from NMR spectra of the ligand and isotopically labeled protein. Using a limited set of 1H-15N backbone amide NMR assignments for MBP and orientational information derived from the RDC measurements in aligned media, an order tensor for MBP has been determined that is consistent with symmetry-based predictions of an axially symmetric system. 13C-1H couplings for a bound trisaccharide ligand, methyl 3,6-di-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside (trimannoside) have been determined at natural abundance and used as orientational constraints. The bound ligand geometry and orientational constraints allowed docking of the trimannoside ligand in the binding site of MBP to produce a structural model for MBP-oligosaccharide interactions.
2.Conformation of a trimannoside bound to mannose-binding protein by nuclear magnetic resonance and molecular dynamics simulations.
Sayers EW1, Prestegard JH. Biophys J. 2002 May;82(5):2683-99.
A model of the carbohydrate recognition domain of the serum form of mannose-binding protein (MBP) from rat complexed with methyl 3,6-di-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside is presented. Allowed conformations for the bound sugar were derived from simulated annealing protocols incorporating distance restraints computed from transferred NOESY spectra. The resulting sugar conformations were then modeled into the MBP binding site, and these models of the complex were refined using molecular dynamics (MD) simulations in the presence of solvent water. These studies indicate that only one of the two major conformations of the alpha(1-->6) linkage found in solution is significantly populated in the bound state (omega = 60 degrees ), whereas the alpha(1-->3) linkage samples at least two states, similar to its behavior in free solution. The bound conformation allows direct hydrogen bonds to form between the sugar and K182 of MBP, in addition to other water-mediated hydrogen bonds.
3.Molecular modeling and NMR studies of benzyl substituted mannosyl trisaccharide binding to two mannose-specific lectins: Allium sativam agglutinin I and Concanavalin A.
Mazumder P1, Mukhopadhyay C. Biopolymers. 2010 Nov;93(11):952-67. doi: 10.1002/bip.21503.
The interaction of trimannoside, α-benzyl 3, 6-di-O-(α-D-mannopyranosyl)-α-D-mannopyranoside, 1 with ASAI (Allium sativam agglutinin I, garlic lectin) was studied to reveal the conformational preferences of this ligand in bound-state and detailed binding mode at atomic level. The binding phenomenon was then compared with another well-known mannose-binding lectin, ConA (Concanavalin A). Structural studies of the ligand in free state were done using NMR spectroscopy and Molecular Dynamics simulations. It is found that the substituted-trimannoside can undergo conformational transitions in solution, with one major and one minor conformation per glycosidic linkage (α 1→3 and α 1→6). On the other hand in the bound-state only one of the two major conformations was significantly populated. The role of phenyl ring in the binding process was explored. An extended binding site was observed for the trimannoside in ASAI utilizing the aromatic substituent, which is not seen in ConA.